Protein kinases have been well documented to play critical roles in almost all important physiological processes of eukaryotes and prokaryotes. They have been extensively used as drug targets to treat various human ailments including cancer. Since, protein kinases play indispensable roles in physiological processes inside a cell, studying their function using conventional gene knock-out approach may not be straight forward. Moreover, knock-down of protein levels using various post-transcriptional and post-translational approaches have their own limitations such as matching the exact timing of the knockdown in enzyme levels to the functional activity inside the cell, residual level of enzyme left and side-effects of the agent used for knock-down.