Hair loss is connected to the loss of stem cells necessary for regular hair creation and hair cycle, and can be caused by a variety of factors including heredity, ageing, hormone imbalances, immunological reactions, and anti-cancer treatment therapies. Medications and autologous hair transplantation are the most common treatments for hair loss, but both have drawbacks, such as the limited effects of drugs and the inability to increase the quantity of hairs in the scalp. A series of interactions between epithelium-derived follicular stem cells and mesenchymal-derived follicular stem cells regulate hair follicle production during embryonic development and postnatal hair cycle. Co-transplantation of these stem cells resulted in efficient production of hair follicles and hairs, according to recent research. Hair shafts are formed when follicular epithelial stem cells differentiate [1]. DP cells send signals to follicular epithelial stem cells that control hair shaft size, form, and colour. Both cell types’ potential to induce hair growth is gradually reduced after isolation from in vivo tissues and throughout expansion culture. As a result, different techniques to maintaining this ability, including as the use of growth factors and signalling molecules, have been investigated. For example, fibroblast growth factor-2 and bone morphogenetic protein eased the loss of DP cell hair induction ability. Fabricating 3D tissues before to transplantation is another option