The highest root regeneration frequency (96.66%) was observed either on MS supplemented with 0.25 mg l-1 NAA or on NN medium with 0.25 mg l-1 IAA. The maximum number of 7.83 roots per shoot with an average root length of 3.98 cm was observed on MS medium supplemented with 0.25 mg l-1 NAA whereas 7.33 roots per shoot with an average root length of 4.13 cm was observed on NN medium supplemented with same level of NAA. Plantlets were acclimatized in garden soil and transferred to field. The method described here can be successfully employed for large scale production of sterile plants for pharmaceutical use and genetic transformation studies.