An efficient protocol is out lined for multiple shoot induction of a medicinally valuable Kaempferia galanga L. using rhizome segment explants. Murashige and Skoog medium supplemented with BA (2.0 mg/ml) and Kn (1.0 mg/ml) exhibited regeneration rate up to 10.85±1.34 shoot/explants. Spontaneous rooting of shoots occurred in the same concentration of cytokinin. The number of leaves (5.21±0.34) and profuse rooting (12.14±1.67) facilitated 100% of plant recovery on acclimation. In vitro derived plants were morphologically identical to the mother plant.