They maintain good personal hygiene, go for regular antenatal services; take adequate food, fruits and vegetables, maintain one sexual partner as a preventive measure for infection during pregnancy. Two major factors which are environment and financial status where identified as the key factors influencing choice of infection preventive behaviour during pregnancy. The analysis of the first hypothesis revealed that there is a significant difference in the infection preventive health behavior during pregnancy among pregnant mothers of childbearing age in Nnamdi Azikiwe University Teaching Hospital Nnewi based on number of gestation and the second hypothesis revealed that there is a significant difference in the infection preventive health behavior during pregnancy among pregnant mothers of childbearing age in Nnamdi Azikiwe University Teaching Hospital Nnewi based on their education level. Based on these findings, it is recommended that the health care provider and pregnant mothers to collaborate towards increasing the level of awareness and adoption of infection preventive health behaviour.