Fusarium venenatum (ATCC 20334) was used for fungal protein production. Date syrup was selected as a carbon source.The critical variables that affected mycoprotein production were identified by Plackett– Burman design (PBD).The significant parameters were the carbon source concentration (10 g/l), temperature (28 �?�?C), seed size (10% v/v), nitrogen and phosphorus sources concentration (3.5 and 1.6 g/l) respectively. The rheological behaviour of filtrated biomass of Fusarium venenatum was studied under steady and dynamic shear conditions.This product showed shear-thinning behaviour with yield stress in the steady shear measurements.With respect to the dynamic shear measurements,the filtrated biomass showed gel-like behaviour.In addition,the viscosity at 100 1/s showed Arrhenius type temperature dependence, with activation energy 2.904 KJ/mol.